Scopus Publication Detail
The publication detail shows the title, authors (with indicators showing other profiled authors), information on the publishing organization, abstract and a link to the article in Scopus. This abstract is what is used to create the fingerprint of the publication.
Ty C. Voss; Ignacio A. Demarco; Richard N. Day (Profiled Author: Richard N. Day)
Over the past decade, genetically encoded fluorescent proteins have become widely used as noninvasive markers in living cells. The development of fluorescent proteins, coupled with advances in digital imaging, has led to the rapid evolution of live-cell imaging methods. These approaches are being applied to address biological questions of the recruitment, co-localization, and interactions of specific proteins within particular subcellular compartments. In the wake of this rapid progress, however, come important issues associated with the acquisition and analysis of ever larger and more complex digital imaging data sets. Using protein localization in the mammalian cell nucleus as an example, we will review some recent developments In the application of quantitative imaging to analyze subcellular distribution and co-localization of proteins in populations of living cells. In this report, we review the principles of acquiring fluorescence resonance energy transfer (FRET) microscopy measurements to define the spatial relationships between proteins. We then discuss how fluorescence lifetime imaging microscopy (FLIM) provides a method that is independent of intensity-based measurements to detect localized protein interactions with spatial resolution. Finally, we consider potential problems associated with the expression of proteins fused to fluorescent proteins for FRET-based measurements from living cells.
PMID: 15786808 PMCID: PMC1237115
This section shows information related to the publication - computed using the fingerprint of the publication - including related publications, related experts with fingerprints representing significant amounts of overlap between their fingerprint and this publication. The red dots indicate whether those experts or terms appear within the publication, thereby showing potential and actual connections.
Richard N. Day; Ignacio A. Demarco; Ty C. Voss; Ye Chen; Ammasi PeriasamyProceedings of SPIE - The International Society for Optical Engineering. 2004;5323:36-43.
A. Periasamy; M. Elangovan; R.N. DayProceedings of SPIE - The International Society for Optical Engineering. 2001;4262:366-372.
R.N. Day; A. PeriasamyProceedings of SPIE - The International Society for Optical Engineering. 2001;4262:163-170.
Appears in this Document