Ahmad R. Safa

School of Medicine, Pharmacology & Toxicology

Scopus Publication Detail

The publication detail shows the title, authors (with indicators showing other profiled authors), information on the publishing organization, abstract and a link to the article in Scopus. This abstract is what is used to create the fingerprint of the publication.

Inhibition of protein synthesis and cell proliferation in cultured human breast cancer cells treated with mitoxantrone

A.R. Safa; M.T. Tseng (Profiled Author: Ahmad R. Safa)

Cancer Letters. 1984;24(3):317-326.

Mitoxantrone suppresses cell proliferation, inhibits protein synthesis and induces ultrastructural alterations in the T-47D and MDA-MB-231 breast cancer cell lines. After 24 h treatment with 10^-9, 10^-7 and 10^-5 M drug and 8 h incubation with [³⁵S]methionine, protein synthesis declined rapidly. While a 10-15% decrease in protein synthesis at 10^-9 M was observed, more than 95% inhibition of protein synthesis occurred at 10^-5 M mitoxantrone in both cell lines. Sodium dodecylsulfate (SDS) gel electrophoresis of labeled proteins revealed no qualitative changes in either cell line. However, only trace amounts of several proteins were present in T-47D cells treated with 10^-5 M drug. At 10^-9 M mitoxantrone had little effect on cell proliferation. At 10^-7 M, 25% and 35% growth inhibition in T-47D and MDA-MB-231 cells was observed, respectively. Cell growth at 10^-5 M was abolished. Cytotoxicity was evident at drug concentrations above 10^-5 M. Ultrastructural alterations in the nucleoli of both cell lines included disintegration and segregation of granular and fibrillar components and the disappearance of nucleolar organizers at 10^-7-10^-5 M mitoxantrone.

PMID: 6498809    

Scientific Context

This section shows information related to the publication - computed using the fingerprint of the publication - including related publications, related experts with fingerprints representing significant amounts of overlap between their fingerprint and this publication. The red dots indicate whether those experts or terms appear within the publication, thereby showing potential and actual connections.