Scopus Publication Detail
The publication detail shows the title, authors (with indicators showing other profiled authors), information on the publishing organization, abstract and a link to the article in Scopus. This abstract is what is used to create the fingerprint of the publication.
Electrophysiological characterization of volume-activated chloride currents in mouse cholangiocyte cell line
Biyi Chen; Grant Nicol; Won Kyoo Cho (Profiled Author: Grant D. Nicol)
American Journal of Physiology - Gastrointestinal and Liver Physiology. 2004;287(6 50-6):G1158-G1167.Abstract
Recent electrophysiological and radioisotope efflux studies have demonstrated various Cl- channels in cholangiocytes including volume-activated Cl- channels (VACC). Because VACCs play prominent roles in many vital cellular functions and physiology in cholangiocytes, we have examined their electrophysiological characteristics in mouse cholangiocytes to provide an important framework for studying in the future. The present study is to characterize VACCs expressed in the mouse bile duct cell (MBDC) line, conditionally immortalized by SV40 virus. Conventional whole cell patch-clamp techniques were used to study the electrophysiological characteristics of VACC in MBDC. When the MBDCs were exposed to hypotonic solution, they exhibited an outwardly rectified current, which was significantly inhibited by replacing chloride in the bath solution with gluconate or glutamate and by administration of classic chloride channel inhibitors 5-nitro-2-(3-phenylpropylamino)-benzoate, glybenclamide, DIDS, and tamoxifen. These inhibitory effects were reversible with washing them out from the bath solution. Moreover, the ion selectivity of the volume-activated channel to different anions indicates that it is more permeable to SCN- > I- ≥ Cl- > F - ≥ acetate ≥ glutamate ≥ gluconate. These electrophysiological characteristics demonstrate that the volume-activated current observed is a VACC. In addition, the VACC in MBDC has electrophysiological characteristics similar to those of the VACC in human cholangiocarcinoma cell line. The present study is the first to characterize the VACC in mouse cholangiocyte and will provide an important framework for further studies to examine and understand the role of the VACC in biliary secretion and ion-transport physiology.
This section shows information related to the publication - computed using the fingerprint of the publication - including related publications, related experts with fingerprints representing significant amounts of overlap between their fingerprint and this publication. The red dots indicate whether those experts or terms appear within the publication, thereby showing potential and actual connections.
Haijun Zhang; Xiaofeng Mei; Pu Zhang; Chao Ma; Fletcher A. White; David F. Donnelly; Robert H. LamotteGLIA. 2009;57(15):1588-1599.
James M. McGill; Margaret S. Yen; Oscar W. Cummings; Gianfranco Alpini; Gene Lesage; Karen E. Pollok; Barbara Miller; Steven K. Engle; Ann P. StansfieldAmerican Journal of Physiology - Gastrointestinal and Liver Physiology. 2001;280(4 43-4):G738-G745.
H.E. Criswell; T.J. McCown; S.S. Moy; G.S. Oxford; R.A. Mueller; A.L. Morrow; G.R. BreeseNeuropharmacology. 1997;36(11-12):1641-1652.
Appears in this Document