The primary structure of the Fab fragment of protein KAU, a monoclonal immunoglobulin M cold agglutinin.

Publication Detail

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J Leoni; J Ghiso; F Goñi; B Frangione (Profiled Author: Frangione, Blas)

Department of Pathology, New York University Medical Center, New York 10016.
The Journal of biological chemistry 1991;266(5):2836-42.

PubMed

Abstract

The complete amino acid sequence of the Fab fragment of protein KAU, a human monoclonal cold agglutinin (IgMk) with anti-I activity, was determined. The light chain (L-chain) consists of 215 residues; the variable (V)L region belongs to the Hum/Kv325/kIIIb sub-subgroup that is preferentially selected in human IgM autoimmune response. The joining (J) region is encoded by the Jk4 gene, and the constant region (C)L domain expresses the km3 allotypic marker. The Fd fragment contains 232 amino acids, and 120 of them comprise the variable domain. The VH region corresponds to the VHIV subgroup and is closely related to the VHIV 2.1 gene isolated from genomic DNA expressed in peripheral blood of a healthy Caucasian. The complementary-determining region 1 has a unique amino acid (Asp) at position 31, and the complementary-determining region 3 codified by the diversity segment (D) gene, shows poor homology with other known D sequences. The joining segment with two unusual substitutions at the D-J junction is encoded by the JH4 gene. Thus, cold agglutinin KAU is an IgM, VkIIIb-Jk4-km3; VHIV-JH4-C mu.

2 Originating Grant

1.

Frangione, Blas

CONFORMATIONAL DISORDERS--AMYLOID AND PRION PROTEINS

1 September 1978 - 31 March 2006
NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES
Total Funding: $ 3,600,812
2.

FRANGIONE, BLAS

STRUCTURE OF IMMUNOGLOBULINS AND RELATED PROTEINS

1 September 1978 - 31 March 1999
NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES
Total Funding: $ 2,819,853

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