Butterfield, D Allan

Publication Detail

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Selective spin labeling of terminal galactose and N-acetylgalactosamine residues on the membrane surface of erythrocytes.

B T Farmer; D A Butterfield (Profiled Author: Butterfield, D Allan)

Journal of biochemical and biophysical methods 1984;10(1-2):111-20.

A method to selectively spin label galactose and N-acetylgalactosamine residues of erythrocyte membrane glycoconjugates is described. The method is based on the activation of the C-6 CH2OH group of these two sugars by galactose oxidase followed by reductive amination with 2,2,6,6-tetramethyl-4-aminopiperidine-1-oxyl in the presence of a mild reducing agent, NaBH3CN. The extent and distribution of the spin labeling suggest that the major sialoglycoprotein, PAS-1, incorporates the greatest amount of spin label while the glycolipids incorporate less than 10% of the spin label.

2 Originating Grant

• 1.

  MARKESBERY, WILLIAM R

  BIOGERONTOLOGY--MULTIDISCIPLINARY

  1 August 1981 - 30 June 1987

  NATIONAL INSTITUTE ON AGING

  Total Funding: $ 0

• 2.

  MARKESBERY, WILLIAM R

  AGING PATHOLOGY IN NEURAL AND EXTRANEURAL TISSUE

  1 April 1981 - 31 March 1985

  NATIONAL INSTITUTE ON AGING

  Total Funding: $ 243,656

Scientific Context

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