The publication detail shows the title, authors (with indicators showing other profiled authors), information on the publishing organization, abstract and a link to the article in PubMed. This abstract is what is used to create the fingerprint of the publication. If any grants are referenced by the publication, they will be listed here as well.
Block of the alpha9 nicotinic receptor by ototoxic aminoglycosides.
C V Rothlin; E Katz; M Verbitsky; D E Vetter; S F Heinemann; A B Elgoyhen (Profiled Author: Ana Elgoyhen)
Instituto de Investigaciones en Ingeniería Genética, Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Ciencias Exactas y Naturales, Vuelta de Obligado 2490, Universidad de Buenos Aires, Argentina.
In the present study, we report that the alpha9 nicotinic acetylcholine receptor (nAChR) expressed in Xenopus laevis oocytes is reversibly blocked by aminoglycoside antibiotics. The aminoglycosides tested blocked the alpha9 nAChR in a concentration-dependent manner with the following rank order of potency: neomycin>gentamicin>streptomycin>amikacin>kanamycin. The antagonistic effect of gentamicin was not overcome by increasing the concentration of acetylcholine (ACh), indicative of a non-competitive type of block. Blockage of ACh-evoked currents by gentamicin was found to be voltage-dependent, being more potent at hyperpolarized than at depolarized holding potentials. Furthermore, gentamicin blockage was dependent upon the extracellular Ca(2+) concentration, shown by the fact that increments in extracellular Ca(2+) significantly reduced the potency of this aminoglycoside to block the alpha9 nAChR. Possible mechanisms of blockage by the aminoglycosides are discussed. The present results suggest that the initial reversible actions of aminoglycosides at the organ of Corti, such as the elimination of the olivocochlear efferent function, are due in part to the interaction with the native alpha9-containing cholinergic receptor of the outer hair cells.
This section shows information related to the publication - computed using the fingerprint of the publication - including related publications, related experts and related grants with fingerprints representing significant amounts of overlap between their fingerprint and this publication. The red dots indicate whether those experts or terms appear within the publication, thereby showing potential and actual connections.
M Verbitsky; C V Rothlin; E Katz; A B ElgoyhenNeuropharmacology 2000;39(13):2515-24.
Murat Oz; Shelley N Jackson; Amina S Woods; Marisela Morales; Li ZhangThe Journal of pharmacology and experimental therapeutics 2005;313(3):1272-80.
Shelley N Jackson; Sachin K Singhal; Amina S Woods; Marisela Morales; Toni Shippenberg; Li Zhang; Murat OzEuropean journal of pharmacology 2008;582(1-3):42-51.
Appears in this Publication
Author of this Publication