The publication detail shows the title, authors (with indicators showing other profiled authors), information on the publishing organization, abstract and a link to the article in PubMed. This abstract is what is used to create the fingerprint of the publication. If any grants are referenced by the publication, they will be listed here as well.
Development of a DNA probe for Ureaplasma urealyticum.
J M Brogan; J Acciai; G L Gallia; F K McCleskey; V G DelVecchio (Profiled Author: Gary Gallia)
Biology Department, University of Scranton, Pennsylvania 18510-4625.
Molecular and cellular probes 1992;6(5):411-6.
Ureaplasma urealyticum has been associated with a variety of disease conditions in humans. However, its exact etiologic role has not been well established because of the difficulties encountered in cultural diagnosis and the time needed for positive identifications. A DNA probe which is specific for a target DNA sequence unique to this suspected pathogen offers a rapid, sensitive and specific means of diagnosis. This study details the development of a polymerase chain reaction system for U. urealyticum. Using conventional hybridization techniques, a cloned genomic fragment was found to be specific for this organism. Sequencing of part of this probe DNA permitted the assignment of oligonucleotide primers which amplified a 186 bp target segment. This PCR system is specific for U. urealyticum but not for other closely related species of mycoplasma. This highly sensitive diagnostic technique will aid in determining the etiologic role, tissue tropism and dynamics of pathogenesis of this organism, and thereby result in better patient care.
This section shows information related to the publication - computed using the fingerprint of the publication - including related publications, related experts and related grants with fingerprints representing significant amounts of overlap between their fingerprint and this publication. The red dots indicate whether those experts or terms appear within the publication, thereby showing potential and actual connections.
Rose M Viscardi; Jennifer Kaplan; Judith C Lovchik; Ju Ren He; Lisa Hester; Srinivas Rao; Jeffrey D HasdayInfection and immunity 2002;70(10):5721-9.
E J Erbelding; T C QuinnDermatologic clinics 1998;16(4):735-8, xi-xii.
F A Rubin; D J Kopecko; R B Sack; P Sudarmono; A Yi; D Maurta; R Meza; M A Moechtar; D C Edman; S L HoffmanThe Journal of infectious diseases 1988;157(5):1051-3.
Appears in this Publication
Author of this Publication