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An analysis of the specificity in pharmacological inhibition of the passive cutaneous anaphylaxis reaction in mice and rats
R.J. Perper; A.L. Oronsky; V. Blancuzzi(Profiled Author: Arnold L. Oronsky)
Journal of Pharmacology and Experimental Therapeutics. 1975;193(2):594-602.Abstract
An antiserum obtained from mice, immunized to produce an antiovalbumin antibody of the IgE type, was employed in a 48 hr passive cutaneous anaphylaxis (PCA) reaction in both mice and rats. The antiserum contained an antibody which, 'fixed' to skin for at least 6 days, was heat labile and eluted from diethylaminoethyl cellulose in the reagin peak. In both rats and mice, the PCA reaction was mediated by a combination of histamine and serotonin and was inhibited by specific antagonists. Various drugs were tested for inhibition of the PCA reaction in recipients also injected with compound 48/80 and histamine. Drugs which have been reported to cause an increase in intracellular cyclic adenosine monophosphate levels [prostaglandins (PG) E
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