The publication detail shows the title, authors (with indicators showing other profiled authors), information on the publishing organization, abstract and a link to the article in PubMed. This abstract is what is used to create the fingerprint of the publication. If any grants are referenced by the publication, they will be listed here as well.
Border cell migration: a model system for live imaging and genetic analysis of collective cell movement.
Mohit Prasad; Xiaobo Wang; Li He; Denise J Montell (Profiled Author: Denise Montell)
Department of Biological Chemistry, Johns Hopkins University School of Medicine, 855 N. Wolfe St, Rangos 456, Baltimore, MD 21205, USA.
Methods in molecular biology (Clifton, N.J.) 2011;769():277-86.
Border cell migration in the Drosophila ovary has emerged as a genetically tractable model for studying collective cell movement. Over many years border cell migration was exclusively studied in fixed samples due to the inability to culture stage 9 egg chambers in vitro. Although culturing late stage egg chambers was long feasible, stage 9 egg chambers survived only briefly outside the female body. We identified culture conditions that support stage 9 egg chamber development and sustain complete migration of border cells ex vivo. This protocol enables one to compare the dynamics of egg chamber development in wild type and mutant egg chambers using time-lapse microscopy and taking advantage of a multiposition microscope with a motorized imaging stage. In addition, this protocol has been successfully used in combination with fluorescence resonance energy transfer biosensors, photo-activatable proteins, and pharmacological agents and can be used with widefield or confocal microscopes in either an upright or inverted configuration.
This section shows information related to the publication - computed using the fingerprint of the publication - including related publications, related experts and related grants with fingerprints representing significant amounts of overlap between their fingerprint and this publication. The red dots indicate whether those experts or terms appear within the publication, thereby showing potential and actual connections.
Lucy X Morris; Allan C SpradlingDevelopment (Cambridge, England) 2011;138(11):2207-15.
Yulia Artemenko; Kristen F Swaney; Peter N DevreotesMethods in molecular biology (Clifton, N.J.) 2011;769():287-309.
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