Divergent T gamma cell functions in antigen-induced blastogenesis: facilitory interactions with Tnon gamma cells and participation in monocyte- and prostaglandin-mediated suppression.

Publication Detail

The publication detail shows the title, authors (with indicators showing other profiled authors), information on the publishing organization, abstract and a link to the article in PubMed. This abstract is what is used to create the fingerprint of the publication. If any grants are referenced by the publication, they will be listed here as well.

M E Kleinhenz; J J Ellner (Profiled Author: Jerrold Ellner)

The Journal of laboratory and clinical medicine 1983;102(5):751-61.

PubMed

Abstract

The contribution of T gamma cells, i.e., T cells bearing surface receptors for the Fc portion of IgG, to the immunologic response to antigen has not been assessed in health or disease. We examined the role of T gamma cells in antigen-induced blastogenesis of T cells from healthy subjects and characterized their participation in monocyte- and prostaglandin-mediated suppression. Cell fractions enriched in the depleted of T gamma cells were prepared from nonadherent T cells by preparative rosetting with IgG-sensitized ox erythrocytes; antigen-induced blastogenesis was assayed as 3H-thymidine incorporation (3H-TdR), by microculture techniques. Removal of T gamma cells resulted in a Tnon gamma cell fraction whose in vitro response to soluble antigen was a mean 40% +/- 8 less than the response of the unseparated T cells from the same donors. Antigen did not induce 3H-TdR in cultures of T gamma cells; however, in cell-mixing experiments, addition of autologous T gamma cells reconstituted the antigen responsiveness of the Tnon gamma cell fraction. Monocytes (MN) added to cell cultures at a ratio known to be suppressive in vitro (MN to T = 1:4) significantly decreased antigen-induced 3H-TdR of unseparated T cells but did not alter the antigen responses of Tnon gamma cells. MN-dependent suppression was abrogated by co-culture with indomethacin. Exogenous prostaglandin E2, an immunosuppressive cyclooxygenase product of MN, selectively decreased antigen-induced 3H-TdR of cell cultures containing T gamma cells but did not affect antigen responses of Tnon gamma cell fraction. Thus these studies show that MN and their cyclooxygenase products modulate T gamma cells to function in a suppressive mode. This demonstration of divergent T gamma cell functions indicates that the contribution of an expanded T gamma cell population to altered antigen reactivity in disease can be determined only by careful functional studies.

Scientific Context

This section shows information related to the publication - computed using the fingerprint of the publication - including related publications, related experts and related grants with fingerprints representing significant amounts of overlap between their fingerprint and this publication. The red dots indicate whether those experts or terms appear within the publication, thereby showing potential and actual connections.