The publication detail shows the title, authors (with indicators showing other profiled authors), information on the publishing organization, abstract and a link to the article in PubMed. This abstract is what is used to create the fingerprint of the publication. If any grants are referenced by the publication, they will be listed here as well.
Detection and quantitation by competitive PCR of an age-associated increase in a 4.8-kb deletion in rat mitochondrial DNA.
W Edris; B Burgett; O C Stine; C R Filburn (Profiled Author: O'Colin Stine)
Laboratory of Biological Sciences, National Institute on Aging, Baltimore, MD 21224.
Mutation research 1994;316(2):69-78.
Recent studies on human tissues have shown that the quantity of partially deleted mitochondrial DNA (mtDNA) increases with age. In this study, mtDNAs from the livers of young adult and old Wistar rats were analyzed by PCR. Evidence for partially deleted mtDNAs was found, with a 4834-bp deletion present in all animals and most easily detected in samples from senescent rats. The deletion breakpoint occurs at a 16-bp direct repeat present in the cytochrome oxidase I and ATPase 6 genes. This deletion in rats is similar in size and location to the 5.0-kb deletion observed in human mtDNA. The proportion of rat mtDNA with this 4.8-kb deletion was quantitated by a competitive PCR assay. The ratio of partially deleted mtDNA/total mtDNA in liver mtDNA from individual 6 month old rats ranged from 5 x 10(-6) to 3 x 10(-5), while the ratio in 24 month old rats ranged from 8 x 10(-4) to 5 x 10(-3), with a mean 100-fold increase with age. These increases are in the range observed for human mtDNA during aging. Thus senescent rats can be used as a model to study this type of mitochondrial DNA damage in aging. The method and reagents described should prove useful in studies of the mechanism(s) underlying deletions, their significance to the aging process, and testing of various compounds or interventions for their ability to slow the process.
This section shows information related to the publication - computed using the fingerprint of the publication - including related publications, related experts and related grants with fingerprints representing significant amounts of overlap between their fingerprint and this publication. The red dots indicate whether those experts or terms appear within the publication, thereby showing potential and actual connections.
Brett G Masayesva; Elizabeth Mambo; Rodney J Taylor; Olga G Goloubeva; Shaoyu Zhou; Yoram Cohen; Khalid Minhas; Wayne Koch; James Sciubba; Anthony J Alberg; et al.Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology 2006;15(1):19-24.
Shuji Nomoto; Montserrant Sanchez-Cespedes; David SidranskyMethods in molecular biology (Clifton, N.J.) 2002;197():107-17.
M P Schoenberg; J M Hakimi; S Wang; G S Bova; J I Epstein; K H Fischbeck; W B Isaacs; P C Walsh; E R BarrackBiochemical and biophysical research communications 1994;198(1):74-80.
Appears in this Publication
Author of this Publication