The publication detail shows the title, authors (with indicators showing other profiled authors), information on the publishing organization, abstract and a link to the article in Scopus. This abstract is what is used to create the fingerprint of the publication.
Interleukin-1 participates in the classical and alternative activation of microglia/macrophages after spinal cord injury
Atsushi Sato; Hirokazu Ohtaki; Tomomi Tsumuraya; Dandan Song; Kenji Ohara; Masahide Asano; Yoichiro Iwakura; Takashi Atsumi; Seiji Shioda (Profiled Author: MASAHIDE ASANO)
Journal of Neuroinflammation. 2012;9.Abstract
Background: Microglia and macrophages (MG/MΦ) have a diverse range of functions depending on unique cytokine stimuli, and contribute to neural cell death, repair, and remodeling during central nervous system diseases. While IL-1 has been shown to exacerbate inflammation, it has also been recognized to enhance neuroregeneration. We determined the activating phenotype of MG/MΦ and the impact of IL-1 in an in vivo spinal cord injury (SCI) model of IL-1 knock-out (KO) mice. Moreover, we demonstrated the contribution of IL-1 to both the classical and alternative activation of MG in vitro using an adult MG primary culture.Methods: SCI was induced by transection of the spinal cord between the T9 and T10 vertebra in wild-type and IL-1 KO mice. Locomotor activity was monitored and lesion size was determined for 14 days. TNFα and Ym1 levels were monitored to determine the MG/MΦ activating phenotype. Primary cultures of MG were produced from adult mice, and were exposed to IFNγ or IL-4 with and without IL-1β. Moreover, cultures were exposed to IL-4 and/or IL-13 in the presence and absence of IL-1β.Results: The locomotor activity and lesion area of IL-1 KO mice improved significantly after SCI compared with wild-type mice. TNFα production was significantly suppressed in IL-1 KO mice. Also, Ym1, an alternative activating MG/MΦ marker, did not increase in IL-1 KO mice, suggesting that IL-1 contributes to both the classical and alternative activation of MG/MΦ. We treated primary MG cultures with IFNγ or IL-4 in the presence and absence of IL-1β. Increased nitric oxide and TNFα was present in the culture media and increased inducible NO synthase was detected in cell suspensions following co-treatment with IFNγ and IL-1β. Expression of the alternative activation markers Ym1 and arginase-1 was increased after exposure to IL-4 and further increased after co-treatment with IL-4 and IL-1β. The phenotype was not observed after exposure of cells to IL-13.Conclusions: We demonstrate here in in vivo experiments that IL-1 suppressed SCI in a process mediated by the reduction of inflammatory responses. Moreover, we suggest that IL-1 participates in both the classical and alternative activation of MG in in vivo and in vitro systems. © 2012 Sato et al; licensee BioMed Central Ltd.
This section shows information related to the publication - computed using the fingerprint of the publication - including related publications, related experts with fingerprints representing significant amounts of overlap between their fingerprint and this publication. The red dots indicate whether those experts or terms appear within the publication, thereby showing potential and actual connections.
Kanako Shichi; Wakako Fujita-Hamabe; Shinichi Harada; Hiroyuki Mizoguchi; Kiyofumi Yamada; Toshitaka Nabeshima; Shogo TokuyamaJournal of Pharmacology and Experimental Therapeutics. 2011;338(2):701-710.
Keiichi Kadoyama; Hiroshi Funakoshi; Wakana Ohya-Shimada; Takahiro Nakamura; Kunio Matsumoto; Shogo Matsuyama; Toshikazu NakamuraNeuroscience Research. 2009;65(2):194-200.
Peirong Lu; Longbiao Li; Gaoqin Liu; Xueguang Zhang; Naofumi MukaidaInvestigative Ophthalmology and Visual Science. 2009;50(10):4761-4768.
Appears in this Document