The publication detail shows the title, authors (with indicators showing other profiled authors), information on the publishing organization, abstract and a link to the article in PubMed. This abstract is what is used to create the fingerprint of the publication. If any grants are referenced by the publication, they will be listed here as well.
Selective recruitment of arrestin-3 to clathrin coated pits upon stimulation of G protein-coupled receptors.
F Santini; R B Penn; A W Gagnon; J L Benovic; J H Keen (Profiled Author: Raymond B Penn)
Department of Microbiology and Immunology and the Kimmel Cancer Institute, Thomas Jefferson University, Philadelphia, PA 19107, USA. email@example.com
Journal of cell science 2000;113 ( Pt 13)():2463-70.
Non-visual arrestins (arrestin-2 and arrestin-3) play critical roles in the desensitization and internalization of many G protein-coupled receptors. In vitro experiments have shown that both non-visual arrestins bind with high and approximately comparable affinities to activated, phosphorylated forms of receptors. They also exhibit high affinity binding, again of comparable magnitude, to clathrin. Further, agonist-promoted internalization of many receptors has been found to be stimulated by exogenous over-expression of either arrestin2 or arrestin3. The existence of multiple arrestins raises the question whether stimulated receptors are selective for a specific endogenous arrestin under more physiological conditions. Here we address this question in RBL-2H3 cells, a cell line that expresses comparable levels of endogenous arrestin-2 and arrestin-3. When (beta)(2)-adrenergic receptors are stably expressed in these cells the receptors internalize efficiently following agonist stimulation. However, by immunofluorescence microscopy we determine that only arrestin-3, but not arrestin-2, is rapidly recruited to clathrin coated pits upon receptor stimulation. Similarly, in RBL-2H3 cells that stably express physiological levels of m1AChR, the addition of carbachol selectively induces the localization of arrestin-3, but not arrestin-2, to coated pits. Thus, this work demonstrates coupling of G protein-coupled receptors to a specific non-visual arrestin in an in vivo setting.
This section shows information related to the publication - computed using the fingerprint of the publication - including related publications, related experts and related grants with fingerprints representing significant amounts of overlap between their fingerprint and this publication. The red dots indicate whether those experts or terms appear within the publication, thereby showing potential and actual connections.
R B Penn; R M Pascual; Y M Kim; S J Mundell; V P Krymskaya; R A Panettieri; J L BenovicThe Journal of biological chemistry 2001;276(35):32648-56.
O B Goodman; J G Krupnick; F Santini; V V Gurevich; R B Penn; A W Gagnon; J H Keen; J L BenovicNature 1996;383(6599):447-50.
O B Goodman; J G Krupnick; F Santini; V V Gurevich; R B Penn; A W Gagnon; J H Keen; J L BenovicAdvances in pharmacology (San Diego, Calif.) 1998;42():429-33.
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