Marcela F Pasetti

Publication Detail

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Attenuated deltaguaBA Salmonella typhi vaccine strain CVD 915 as a live vector utilizing prokaryotic or eukaryotic expression systems to deliver foreign antigens and elicit immune responses.

M F Pasetti; R J Anderson; F R Noriega; M M Levine; M B Sztein (Profiled Authors: Myron M Levine; Marcela F Pasetti; Marcelo B Sztein)

Department of Pediatrics, University of Maryland, School of Medicine, Baltimore, Maryland 21201, USA.
Clinical immunology (Orlando, Fla.) 1999;92(1):76-89.

Attenuated Salmonella typhi strain CVD 915, harboring a deletion in guaBA that interrupts the biosynthesis of guanine nucleotides, was evaluated as a live vector vaccine for delivering foreign antigens utilizing prokaryotic or eukaryotic expression systems. Plasmids pTETnir15 and pcDNA3tetC encoding fragment C (Frag C) of tetanus toxin under the control of prokaryotic or eukaryotic promoters, respectively, were introduced into CVD 915 and administered intranasally to mice. Purified pcDNA3tetC and Frag C were given intramuscularly. High titers of serum IgG1, IgG2a, and IgG2b antibodies against Frag C were elicited by CVD 915(pTETnir15) and CVD 915(pcDNA3tetC). These responses were significantly higher than those induced by pcDNA3tetC. Proliferative responses and IL-2 and IFN-gamma production were observed in splenocytes exposed to S. typhi antigens and Frag C. We conclude that CVD 915 is a highly efficient live vector to carry foreign genes under eukaryotic or prokaryotic control and elicit potent immune responses.

4 Originating Grant

• 1.

  Levine, Myron Max

  BACTERIAL LIVE VECTOR-BASED VACCINE AGAINST MALARIA

  1 April 1997 - 31 May 2006

  NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES

• 2.

  SZTEIN, MARCELO B.

  Immune Mechanisms of Protection in S Typhi Vaccines

  1 September 1994 - 30 November 2011

  NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES

• 3.

  Levine, Myron Max

  Recombinant & live oral Salmonella typhi hybrid vaccines

  1 April 1990 - 28 February 2009

  NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES

• 4.

  LEVINE, MYRON M

  RECOMBINANT & LIVE ORAL SALMONELLA TYPHI HYBRID VACCINES

Scientific Context

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