Gary M Fiskum

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ATP synthesis is coupled to rat liver mitochondrial RNA synthesis.

S F DasGupta; S I Rapoport; M Gerschenson; E Murphy; G Fiskum; S J Russell; K Chandrasekaran (Profiled Author: Gary M Fiskum)

Section on Brain Physiology and Metabolism, NIA, NIH, Bethesda, MD, USA.
Molecular and cellular biochemistry 2001;221(1-2):3-10.

Rat liver mitochondria respond to changes in energy demand by modulating the amount of RNA synthesized. Coupled rat liver mitochondria were used to determine the relationship between mitochondrial respiration, ATP levels, and mitochondrial transcription. This system included oxidizable substrates (malate and glutamate) and constituents that could support both mitochondrial respiration and transcription. The respiratory inhibitor rotenone, phosphorylation inhibitor oligomycin, and the uncoupler of oxidative phosphorylation carbonyl-cyanide p-triflouromethoxyphehylhydrazone inhibited RNA synthesis. Addition of ADP stimulated mitochondrial transcription and peak RNA synthesis was observed at 1-2 mM ADP. At ADP concentrations above 2 mM, RNA synthesis decreased. These results demonstrate that mitochondrial transcription is tightly coupled to ATP levels.

1 Originating Grant

• 1.

  Fiskum, Gary

  Molecular Mechanisms of Ischemia Reperfusion Brain Injury

  1 May 1995 - 31 December 2008

  NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE

Scientific Context

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