Publication Detail

The publication detail shows the title, authors (with indicators showing other profiled authors), information on the publishing organization, abstract and a link to the article in PubMed. This abstract is what is used to create the fingerprint of the publication. If any grants are referenced by the publication, they will be listed here as well.

Effect of plasmonic nanostructures and nanofilms on fluorescence resonance energy transfer.

Henryk Szmacinski; Krishanu Ray; Joseph R Lakowicz (Profiled Authors: Joseph R Lakowicz; Krishanu Ray; Henryk Szmacinski)

Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA. henry@cfs.umbi.umd.edu
Journal of biophotonics 2009;2(4):243-52.

We investigate the effect of silver nanostructures and nanofilms on fluorescence resonance energy transfer (FRET). Thin layers of poly (vinyl alcohol) of 10 or 20 nm with homogenously distributed donor (Cy3) and acceptor (Cy5) molecules were coated on silver islands and continuous silver films. Several geometrical configurations were studied with multiple donor-acceptor (D-A) samples with various acceptor concentrations. The acceptor concentrations were adjusted to yield FRET efficiencies from about 10 to 92% on bare glass surfaces. Time-resolved fluorescence spectroscopy was used to determine the energy transfer efficiency and the relative transfer rate. It was found that the relative transfer rate increased 2-fold in the presence of continuous silver films and about 25-fold in presence of silver island films. The largest enhancements in the energy transfer efficiency, up to 90%, were observed for low acceptor concentrations (large donor-acceptor separations). To a first approximation, observed changes in FRET efficiencies were explained by an increase of the quantum yield of the donor molecules in the presence of silver islands.

2 Originating Grant

• 1.

  LAKOWICZ, JOSEPH R.

  Plasmon-Controlled Fluorescence and Cardiac Markers

  1 June 2006 - 31 July 2016

  NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING

• 2.

  LAKOWICZ, JOSEPH R.

  Metallic Surfaces and Particles in DNA Analysis

  27 June 2003 - 30 June 2013

  NATIONAL HUMAN GENOME RESEARCH INSTITUTE

Scientific Context

This section shows information related to the publication - computed using the fingerprint of the publication - including related publications, related experts and related grants with fingerprints representing significant amounts of overlap between their fingerprint and this publication. The red dots indicate whether those experts or terms appear within the publication, thereby showing potential and actual connections.