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Culture human mesenchymal stem cells with calcium phosphate cement scaffolds for bone repair.
Michael D Weir; Hockin H K Xu (Profiled Author: Huakun Xu)
Department of Endodontics, Prosthodontics and Operative Dentistry, Dental School, University of Maryland, Baltimore, MD 21201, USA.
Journal of biomedical materials research. Part B, Applied biomaterials 2010;93(1):93-105.
Because of its moldability and excellent osteoconductivity, calcium phosphate cement (CPC) is highly promising for craniofacial and orthopedic applications. The objectives of this study were to investigate the response of human mesenchymal stem cells (hMSCs) to a high-strength CPC-chitosan scaffold and to examine cell proliferation and osteogenic differentiation. hMSCs were seeded onto CPC-chitosan composite, CPC control, and tissue culture polystyrene (TCPS). Alkaline phosphatase activity (ALP) and mineralization of hMSCs were measured. CPC-chitosan had a flexural strength (mean + or - SD; n = 5) of (19.5 + or - 1.4) MPa, higher than (8.0 + or - 1.4) MPa of CPC control (p < 0.05). The percentage of live hMSCs on CPC-chitosan was (90.5 + or - 1.3)% at 8 days, matching (90.7 + or - 3.8)% of CPC control (p > 0.1). The CPC-chitosan surface area covered by the attached hMSCs increased from (51 + or - 11)% at 1 day to (90 + or - 4)% at 8 days (p < 0.05), matching those of CPC control (p > 0.1). Hence, the CPC strength was significantly increased via chitosan without compromising the hMSC response. At 8 days, there was a significant increase in ALP of cells in osteogenic media (10.99 + or - 0.93) [(mM pNpp/min)/(microg DNA)] versus control media (3.62 + or - 0.40) (p < 0.05). hMSCs in osteogenic media exhibited greater mineralization area of (47.5 + or - 19.7)% compared with (6.1 + or - 2.3)% in control medium on TCPS (p < 0.05). In conclusion, hMSCs showed excellent attachment and viability on the strong and tough CPC-chitosan scaffold, matching the hMSC response on CPC control. hMSCs were successfully differentiated down the osteogenic lineage. Hence, the strong, in situ hardening CPC-chitosan scaffold may be useful as a moderate load-bearing vehicle to deliver hMSCs for maxillofacial and orthopedic bone tissue engineering.
2 Originating Grant
1 April 2008 - 31 March 2012
NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH
1 July 2001 - 31 January 2014
NATIONAL INSTITUTE OF DENTAL &CRANIOFACIAL RESEARCH
This section shows information related to the publication - computed using the fingerprint of the publication - including related publications, related experts and related grants with fingerprints representing significant amounts of overlap between their fingerprint and this publication. The red dots indicate whether those experts or terms appear within the publication, thereby showing potential and actual connections.
H H K Xu; L Zhao; M D WeirJournal of dental research 2010;89(12):1482-8.
Liang Zhao; Michael D Weir; Hockin H K XuBiomaterials 2010;31(14):3848-57.
Michael D Weir; Hockin H K XuActa biomaterialia 2010;6(10):4118-26.
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