Publication Detail

The publication detail shows the title, authors (with indicators showing other profiled authors), information on the publishing organization, abstract and a link to the article in PubMed. This abstract is what is used to create the fingerprint of the publication. If any grants are referenced by the publication, they will be listed here as well.

Human umbilical cord stem cell encapsulation in calcium phosphate scaffolds for bone engineering.

Liang Zhao; Michael D Weir; Hockin H K Xu (Profiled Author: Huakun Xu)

Department of Endodontics, Prosthodontics and Operative Dentistry, University of Maryland Dental School, Baltimore, MD 21201, USA.
Biomaterials 2010;31(14):3848-57.

Human bone marrow mesenchymal stem cells (hBMSCs) require an invasive procedure to harvest, and have lower self-renewal potential with aging. Umbilical cord mesenchymal stem cells (hUCMSCs) are a relatively new stem cell source; this study reveals a self-setting and load-bearing calcium phosphate construct that encapsulates these stem cells. The flexural strength (mean+/-sd; n=5) of the hUCMSC-encapsulating calcium phosphate cement (CPC) increased from (3.5+/-1.1) MPa without polyglactin fibers, to (11.7+/-2.1) MPa with 20% of polyglactin fibers (p<0.05). hUCMSCs attached to the bone mineral-mimicking scaffold in the osteogenic media and differentiated down the osteogenic lineage, yielding elevated alkaline phosphatase (ALP) and osteocalcin (OC) gene expressions. ALP and OC on the CPC-fiber scaffold was 2-fold those on CPC control without fibers. hUCMSCs encapsulated inside the scaffolds retained excellent viability and cell density. The encapsulated hUCMSCs inside four different constructs successfully differentiated down the osteogenic lineage and synthesized bone minerals, as confirmed by mineral staining, SEM, and XRD. The percentage of mineral area synthesized by the encapsulated hUCMSCs increased from about 3% at day-7, to 12% at day-21 (p<0.05). In conclusion, this study demonstrated that hUCMSCs encapsulated in the bioengineered scaffolds osteo-differentiated and synthesized bone minerals. The self-setting CPC-chitosan-fiber scaffold supported the viability and osteogenic differentiation of the encapsulated hUCMSCs, and had mechanical strength matching that of cancellous bone.

2 Originating Grant

• 1.

  XU, HUAKUN

  Development of high performance, caries-inhibiting dental nano-materials

  1 April 2008 - 31 March 2012

  NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH

• 2.

  XU, HUAKUN

  Injectable and Strong Nano-Apatite/Stem Cell Scaffolds for Bone Regeneration

  1 July 2001 - 31 January 2014

  NATIONAL INSTITUTE OF DENTAL &CRANIOFACIAL RESEARCH

Scientific Context

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