J Marc Simard

Publication Detail

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The patch clamp technique.

L K Liem; J M Simard; Y Song; K Tewari (Profiled Author: J Marc Simard)

Division of Neurosurgery, University of Maryland School of Medicine, University of Maryland Medical Center, Baltimore, USA.
Neurosurgery 1995;36(2):382-92.

The introduction of the patch clamp technique less than two decades ago revolutionized the study of cellular physiology by providing a high-resolution method of observing the function of individual ionic channels in a variety of normal and pathological cell types. By the use of variations of the basic recording methodology, cellular function and regulation can be studied at a molecular level by observing currents through individual ionic channels. At a cellular level, processes such as signaling, secretion, and synaptic transmission can be examined. In addition, by combining the information from high-resolution electrophysiological recordings obtained by the patch clamp method with modern molecular biological techniques, further insight can be gained into the gene expression and protein structure of ionic channels. Given the ubiquity and importance of ionic channels, it is not surprising that their study has led to a new understanding of the mechanisms of certain disease processes and has given insight into treatments for these diseases. This review gives an historical perspective of the development of the patch clamp technique and an overview of the methodologies currently in use. Examples are shown to illustrate typical uses of the patch clamp technique with emphasis on the variety of recording configurations available and the advantages and drawbacks of each method.

2 Originating Grant

• 1.

  Simard, Jmarc

  Cerebrovascular Ion Channels in Hypertension

  1 April 1994 - 29 February 2008

  NATIONAL HEART, LUNG, AND BLOOD INSTITUTE

• 2.

  SIMARD, J. M

  IONIC CHANNELS IN CEREBROVASCULAR SMOOTH MUSCLE CELLS

  1 April 1990 - 31 March 1996

  NATIONAL HEART, LUNG, AND BLOOD INSTITUTE

Scientific Context

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