Preetha Ramalingam

Pathology, Pathology/Lab Medicine

Scopus Publication Detail

The publication detail shows the title, authors (with indicators showing other profiled authors), information on the publishing organization, abstract and a link to the article in Scopus. This abstract is what is used to create the fingerprint of the publication.

Calretinin immunostaining as an adjunct in the diagnosis of Hirschsprung disease

Susan K. Holland; Preetha Ramalingam; Robert H. Podolsky; Michelle D. Reid-Nicholson; Jeffrey R. Lee (Profiled Author: Preetha Ramalingam)

Annals of Diagnostic Pathology. 2011;15(5):323-328.

Historically, the diagnosis of Hirschsprung disease was made by evaluating multiple hematoxylin and eosin-stained slides and performing acetylcholinesterase histochemical staining. Recently, calretinin immunohistochemical staining has been reported and found to be superior to acetylcholinesterase staining in the confirmation of aganglionosis. We retrieved tissue blocks from 23 patients with proven Hirschsprung disease from the archives of the Medical College of Georgia. In addition, we selected 23 control patients with ganglion cells. All cases were stained with calretinin, and the presence or absence of both intrinsic nerve fibers (INFs) and ganglion cells was scored by 4 pathologists with fairly strong agreement (κ = 0.858). All cases of proven Hirschsprung disease were negative for INFs. Eighty-three percent of non-Hirschsprung patients were positive for INFs. Based on statistical analysis, the association between disease status and pathologist rating was statistically significant (P <.0001). We also found calretinin immunostaining to be a useful adjunctive modality in the diagnosis of Hirschsprung disease. © 2011 Elsevier Inc.

PMID: 21641840    

Scientific Context

This section shows information related to the publication - computed using the fingerprint of the publication - including related publications, related experts with fingerprints representing significant amounts of overlap between their fingerprint and this publication. The red dots indicate whether those experts or terms appear within the publication, thereby showing potential and actual connections.