Eleanor G Rogan

University of Nebraska Medical Center, Environmental, Agricultural & Occupational Health Sciences

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Determination of benzo[a]pyrene- and 7,12-dimethylbenz[a]anthracene-DNA adducts formed in rat mammary glands

Rosa Todorovic; Freek Ariese; Prabhakar Devanesan; Ryszard Jankowiak; Gerald J. Small; Eleanor Rogan; Ercole Cavalieri (Profiled Authors: Ercole Cavalieri; Eleanor G Rogan)

Chemical Research in Toxicology 1997;10(9):941-947.

Both 7,12-dimethylbenz[a]anthracene (DMBA) and benzo[a]pyrene (BP) are carcinogenic in the rat mammary gland. The depurinating and stable adducts of DMBA and BP formed in vitro and in mouse skin were previously identified and quantitated. Identification and quantitation of the depurinating and stable DNA adducts of DMBA and identification of the depurinating adducts of BP formed in rat mammary glands in the 24 h after intramammillary injection of DMBA or BP are reported in this paper. The depurinating adducts of DMBA, which constitute 52% of all adducts detected, are DMBA bound at the 12- methyl group to the N-7 of adenine (Ade) or guanine (Gua), namely, 7- methylbenz[a]anthracene (MBA)-12-CH 2 -N7Ade (39%) and 7-MBA-12-CH 2 -N7Gua (13%). All of the stable adducts were formed from the diol epoxide(s) of DMBA. Depurinating adducts of BP with guanine, namely, 8-(BP-6-yl)-guanine (BP-6-C8Gua) and BP-6-N7Gua, were identified in rat mammary glands treated with BP. The major stable adduct, formed via the diol epoxide pathway, BP- diol epoxide-10-N 2 dG, accounted for over 64% of all the stable adducts. Three other BP-DNA stable adducts remain unidentified. Thus, rat mammary cells form depurinating adducts of DMBA and BP predominantly via their radical cations and stable adducts via the diol epoxides.

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