Manage your Funding Opportunities
  

Holly Simon Institute of Environmental Health

Empty picture place holder

Holly Simon

Office phone

(503) 418-8391

Email

Manage your Funding

Scopus Publication Detail

The publication detail shows the title, authors (with indicators showing other profiled authors), information on the publishing organization, abstract and a link to the article in Scopus. This abstract is what is used to create the fingerprint of the publication.


Development of real-time assays for impedance-based detection of microbial double-stranded DNA targets: Optimization and data analysis

Andrei L. Ghindilis; Maria W. Smith; Dean S. Messing; Vena N. Haynes; George B. Middleton; Kevin R. Schwarzkopf; Carmen E. Campbell; Changqing Zhan; Bruce Ulrich; Michael J. Frasier; et al.

(Profiled Author: Holly Simon)

Biosensors and Bioelectronics. 2012;35(1):87-93.

Abstract

A real-time, label free assay was developed for microbial detection, utilizing double-stranded DNA targets and employing the next generation of an impedimetric sensor array platform designed by Sharp Laboratories of America (SLA). Real-time curves of the impedimetric signal response were obtained at fixed frequency and voltage for target binding to oligonucleotide probes attached to the sensor array surface. Kinetic parameters of these curves were analyzed by the integrated data analysis package for signal quantification. Non-specific binding presented a major challenge for assay development, and required assay optimization. For this, differences were maximized between binding curve kinetic parameters for probes binding to complementary targets versus non-target controls. Variables manipulated for assay optimization included target concentration, hybridization temperature, buffer concentration, and the use of surfactants. Our results showed that (i) different target-probe combinations required optimization of specific sets of variables; (ii) for each assay condition, the optimum range was relatively narrow, and had to be determined empirically; and (iii) outside of the optimum range, the assay could not distinguish between specific and non-specific binding. For each target-probe combination evaluated, conditions resulting in good separation between specific and non-specific binding signals were established, generating high confidence in the SLA impedimetric dsDNA assay results. © 2012 Elsevier B.V.


PMID: 22405908    

Scientific Context

This section shows information related to the publication - computed using the fingerprint of the publication - including related publications, related experts with fingerprints representing significant amounts of overlap between their fingerprint and this publication. The red dots indicate whether those experts or terms appear within the publication, thereby showing potential and actual connections.

Related Publications

Related Topics

Appears in this Publication Appears in this Document

Related Experts

Author of this Publication Author of this Document