Manage your Funding Opportunities
  

Peter Zuber Institute of Environmental Health

Empty picture place holder

Peter Zuber

Office phone

(503) 494-0584

Email

Manage your Funding

Scopus Publication Detail

The publication detail shows the title, authors (with indicators showing other profiled authors), information on the publishing organization, abstract and a link to the article in Scopus. This abstract is what is used to create the fingerprint of the publication.


Evidence that a single monomer of Spx can productively interact with RNA polymerase in Bacillus subtilis

Ann A. Lin; Peter Zuber

(Profiled Author: Peter Zuber)

Journal of Bacteriology. 2012;194(7):1697-1707.

Abstract

Spx activates transcription initiation in Bacillus subtilis by directly interacting with the C-terminal domain of the RNA polymerase(RNAP) holoenzyme- subunit, which generates a complex that recognizes the promoter regions of genes within the Spxregulon. Many Gram-positive species possess multiple paralogs of Spx, suggesting that two paralogous forms of Spx could simultaneouslycontact RNAP. The composition of Spx/RNAP was examined in vitro using an Spx variant (SpxδCHA) bearing a 12-amino-acid deletion of the C terminus (SpxδC) and a hemagglutinin (HA) epitope tag and Spxc-Myc, a full-length Spx with aC-terminal myelocytomatosis oncoprotein (c-Myc) epitope tag. All Spx/RNAP complexes bearing deletion or C-terminal-taggedvariants were transcriptionally active in vivo and in vitro. Reaction mixtures containing SpxδCHA and Spxc-Myc combinedwith RNAP were applied to either anti-HA or anti-c-Myc affinity columns. Eluted fractions contained RNAP with only one of theepitope-tagged Spx derivatives. The resin-bound RNAP complex bearing a single epitope-tagged Spx derivative was transcriptionallyactive. In vivo production of SpxδC and SpxδCHA followed by anti-HA affinity column chromatography of a clearedlysate resulted in retrieval of Spx/RNAP with only the SpxδCHA derivative. Binding reactions that combined active Spxc-Myc, inactive Spx(R60E)δCHA, and RNAP, when applied to the anti-HA affinity column, yielded only inactive Spx(R60E)δCHA/RNAP complexes. The results strongly argue for a model in which a single Spx monomer engages RNAP to generate an activetranscriptional complex. © 2012, American Society for Microbiology.


PMID: 22307755     PMCID: PMC3302468    

Scientific Context

This section shows information related to the publication - computed using the fingerprint of the publication - including related publications, related experts with fingerprints representing significant amounts of overlap between their fingerprint and this publication. The red dots indicate whether those experts or terms appear within the publication, thereby showing potential and actual connections.

Related Publications

Related Topics

Appears in this Publication Appears in this Document

Related Experts

Author of this Publication Author of this Document