Scopus Publication Detail
The publication detail shows the title, authors (with indicators showing other profiled authors), information on the publishing organization, abstract and a link to the article in Scopus. This abstract is what is used to create the fingerprint of the publication.
In vitro development of secondary follicles from cryopreserved rhesus macaque ovarian tissue after slow-rate freeze or vitrification
Alison Y. Ting; Richard R. Yeoman; Maralee S. Lawson; Mary B. Zelinski (Profiled Author: Mary Zelinski)
Human Reproduction. 2011;26(9):2461-2472.Abstract
Background Ovarian tissue cryopreservation is the only option for preserving fertility in prepubertal girls and cancer patients requiring immediate treatment. Following ovarian tissue cryopreservation, fertility can be restored after tissue transplant or in vitro follicle maturation. Methods Macaque (n = 4) ovarian cortex was cryopreserved using slow-rate freezing (slow freezing) or vitrification. Tissues were fixed for histology or phosphohistone H3 (PPH3) analysis, cultured with bromodeoxyuridine (BrdU) or used for three-dimensional secondary follicle culture. Follicular diameter and steroid hormones were measured weekly. Results Slow freezing induced frequent cryo-injuries while vitrification consistently maintained morphology of the stroma and secondary follicles. PPH3 was similar in fresh and vitrified, but sparse in slow-frozen tissues. BrdU uptake appeared diminished following both Methods compared with that in fresh follicles. In vitro follicle survival and growth were greater in fresh than in cryopreserved follicles. Antrum formation appeared similar after vitrification compared with the fresh, but was reduced following slow freezing. Steroid production was delayed or diminished following both Methods compared with fresh samples. Conclusions Secondary follicle morphology was improved after vitrification relative to slow freezing. Following vitrification, stroma was consistently more compact with intact cells typical to that of fresh tissue. BrdU uptake demonstrated follicle viability post-thaw/warming. For the first time, although not to the extent of fresh follicles, macaque follicles from cryopreserved tissue can survive, grow, form an antrum and produce steroid hormones, indicating some functional preservation. The combination of successful ovarian tissue cryopreservation with in vitro maturation of follicles will offer a major advancement to the field of fertility preservation. © 2011 The Author.
PMID: 21705370 PMCID: PMC3157627
This section shows information related to the publication - computed using the fingerprint of the publication - including related publications, related experts with fingerprints representing significant amounts of overlap between their fingerprint and this publication. The red dots indicate whether those experts or terms appear within the publication, thereby showing potential and actual connections.
Shiying Jin; Lei Lei; Lonnie D. Shea; Mary B. Zelinski; Richard L. Stouffer; Teresa K. WoodruffFertility and Sterility. 2010;93(8):2627-2632.
Alison Y. Ting; Richard R. Yeoman; Maralee S. Lawson; Mary B. ZelinskiCryobiology. 2012;65(1):1-11.
Jay W. Wright; Suellen Toth-Fejel; Richard L. Stouffer; Karin D. RodlandEndocrinology. 2002;143(6):2198-2207.
Appears in this Document