Orthopedics

Scopus Publication Detail

The publication detail shows the title, authors (with indicators showing other profiled authors), information on the publishing organization, abstract and a link to the article in Scopus. This abstract is what is used to create the fingerprint of the publication.

Physical and functional interaction between ribosomal protein L11 and the tumor suppressor ARF

Mu-Shui Dai; Kishore B. Challagundla; Xiao-Xin Sun; Lakshmi Reddy Palam; Shelya X. Zeng; Ronald C. Wek; Hua Lu (Profiled Author: Mushui Dai)

Journal of Biological Chemistry. 2012;287(21):17120-17129.

The ARF tumor suppressor protein activates p53 in response to oncogenic stress, whereas ribosomal protein L11 induces p53 following ribosomal stress. Both proteins bind to central, albeit non-overlapping, regions of MDM2 and suppress MDM2 activity toward p53. However, it is not known whether the two pathways are functionally connected. Here we show that ARF directly binds to L11 in vitro and in cells, which then forms a complex with MDM2 and p53. L11 collaboratively enhances ARF-induced p53 transcriptional activity and cell cycle arrest. Supporting these results, knocking down L11 reduces ARF-mediated p53 accumulation and alleviates ARF-induced cell cycle arrest. Interestingly, overexpression of ARF increases the levels of ribosome-free L11 and enhances the interaction of L11 with MDM2 and p53. These results demonstrate that ARF activates p53, at least partly by induction of ribosomal stress, which results in L11 suppression of MDM2, and suggest that the ARFMDM2-p53 and the L11-MDM2-p53 pathways are functionally connected. © 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

PMID: 22467867     PMCID: PMC3366788

Scientific Context

This section shows information related to the publication - computed using the fingerprint of the publication - including related publications, related experts with fingerprints representing significant amounts of overlap between their fingerprint and this publication. The red dots indicate whether those experts or terms appear within the publication, thereby showing potential and actual connections.