Barbara R Grubb

UNCCH, School of Medicine, Medicine

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Ion transport across the normal and CF neonatal murine intestine

B.R. Grubb (Profiled Author: Barbara R Grubb)

American Journal of Physiology - Gastrointestinal and Liver Physiology. 1999;277(1 40-1):G167-G174.

Neonatal mice with cystic fibrosis (CF) exhibit a very high mortality due to intestinal obstruction localized primarily to the ileum and colon. It has been hypothesized that lack of Cl^- secretion and possibly elevated Na⁺ absorption contribute to the gut problems in CF neonates. Therefore, intestines (ileum, proximal colon, and distal colon) from normal and CF day- old mouse pups were studied on ultra-small-aperture (0.0135 cm²) Ussing chambers. All three regions of the normal neonatal intestine responded to forskolin with an increase in short-circuit current, which was completely absent in the CF intestine. The neonatal distal colon exhibited a high rate of amiloride-sensitive electrogenic Na⁺ absorption, which did not differ between the normal and CF preparations. The ileum and proximal colon of both genotypes exhibited a small but significant electrogenic Na⁺ absorption. The neonatal proximal colon and ileum also exhibited electrogenic Na⁺-glucose cotransport, which was significantly greater in the normal compared with the CF ileum. In addition, all three intestinal regions exhibited electrogenic Na⁺-alanine cotransport, which was significantly reduced in two of the regions of the CF neonatal intestine. It is speculated that: 1) the reduced rate of Na⁺-nutrient cotransport in the CF intestine contributes to the lower rate of growth in CF pups, whereas 2) the elevated electrogenic Na⁺ absorption in the neonatal intestine, coupled with an inability to secrete Cl^-, contributes to the intestinal obstruction in the CF pups.

PMID: 10409164    

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