Mitsuo Yamauchi

UNCCH, School of Dentistry, Periodontology

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Post-translational modifications of collagen upon BMP-induced osteoblast differentiation

Masaru Kaku; Yoshiyuki Mochida; Phimon Atsawasuwan; Duenpim Parisuthiman; Mitsuo Yamauchi (Profiled Author: Mitsuo Yamauchi)

Biochemical and Biophysical Research Communications. 2007;359(3):463-468.

The pattern of collagen cross-linking is tissue specific primarily determined by the extent of hydroxylation and oxidation of specific lysine residues in the molecule. In this study, murine pre-myoblast cell line, C2C12 cells, were transdifferentiated into osteoblastic cells by bone morphogenetic protein (BMP)-2 treatment, and the gene expression of lysyl hydroxylases (LH1, 2a/b, and 3) and lysyl oxidase (LOX)/lysyl oxidase-like proteins (LOXL1-4), and the extent of hydroxylysine were analyzed. After 24 h of treatment, the expression of most isoforms were upregulated up to 96 h whereas LH2a and LOXL2 decreased with time. In the treated cells, both hydroxyproline and hydroxylysine were detected at day 7 and increased at day 14. The ratio of hydroxylysine to hydroxyproline was significantly increased at day 14. The results indicate that LHs and LOX/LOXLs are differentially responsive to BMP-induced osteoblast differentiation that may eventually lead to the specific collagen cross-linking pattern seen in bone. © 2007 Elsevier Inc. All rights reserved.

PMID: 17553463     PMCID: PMC1994940

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