Scopus Publication Detail
The publication detail shows the title, authors (with indicators showing other profiled authors), information on the publishing organization, abstract and a link to the article in Scopus. This abstract is what is used to create the fingerprint of the publication.
T. Wang; R.V. Campbell; M.K. Yi; S.M. Lemon; S.A. Weinman (Profiled Author: Stanley M Lemon)
Journal of viral hepatitis. 2010;17(11):784-793.Abstract
Hepatitis C virus (HCV) infection results in several changes in mitochondrial function including increased reactive oxygen species (ROS) production and greater sensitivity to oxidant, Ca(2+) and cytokine-induced cell death. Prior studies in protein over-expression systems have shown that this effect can be induced by the core protein, but other viral proteins and replication events may contribute as well. To evaluate the specific role of core protein in the context of viral replication and infection, we compared mitochondrial sensitivity in Huh7-derived HCV replicon bearing cells with or without core protein expression with that of cells infected with the JFH1 virus strain. JFH1 infection increased hydrogen peroxide production and sensitized cells to oxidant-induced loss of mitochondrial membrane potential and cell death. An identical phenomenon occurred in genome-length replicons-bearing cells but not in cells bearing the subgenomic replicons lacking core protein. Both cell death and mitochondrial depolarization were Ca(2+) dependent and could be prevented by Ca(2+) chelation. The difference in the mitochondrial response of the two replicon systems could be demonstrated even in isolated mitochondria derived from the two cell lines with the 'genome-length' mitochondria displaying greater sensitivity to Ca(2+) -induced cytochrome c release. In vitro incubation of 'subgenomic' mitochondria with core protein increased oxidant sensitivity to a level similar to that of mitochondria derived from cells bearing genome-length replicons. These results indicate that increased mitochondrial ROS production and a reduced threshold for Ca(2+) and ROS-induced permeability transition is a characteristic of HCV infection. This phenomenon is a direct consequence of core protein interactions with mitochondria and is present whenever core is expressed, either in infection, full-length replicon-bearing cells, or in over-expression systems. © 2009 Blackwell Publishing Ltd.
PMID: 20002299 PMCID: PMC2970657
This section shows information related to the publication - computed using the fingerprint of the publication - including related publications, related experts with fingerprints representing significant amounts of overlap between their fingerprint and this publication. The red dots indicate whether those experts or terms appear within the publication, thereby showing potential and actual connections.
Michael Kovochich; Benjamin Espinasse; Melanie Auffan; Ernest M. Hotze; Lauren Wessel; Tian Xia; Andre E. Nel; Mark R. WiesnerEnvironmental Science and Technology. 2009;43(16):6378-6384.
Michiari Okuda; Kui Li; Michael R. Beard; Lori A. Showalter; Frank Scholle; Stanley M. Lemon; Steven A. WeinmanGastroenterology. 2002;122(2):366-375.
Shuang Mei; Haihua Gu; Xuefeng Yang; Huailan Guo; Zhenqi Liu; Wenhong CaoEndocrinology. 2012;153(5):2120-2129.
Appears in this Document