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Structure and Ca2+-binding properties of the tandem C 2 Domains of E-Syt2

Junjie Xu; Taulant Bacaj; Amy Zhou; Diana R. Tomchick; Thomas C. Südhof; Josep Rizo

(Profiled Authors: Jose Rizo-Rey; Diana R Tomchick)

Structure. 2014;22(2):269-280.


Contacts between the endoplasmic reticulum and the plasma membrane involve extended synaptotagmins (E-Syts) in mammals or tricalbins in yeast, proteins with multiple C2 domains. One of the tandem C2 domains of E-Syt2 is predicted to bind Ca2+, but no Ca2+-dependent function has been attributed to this protein. We have determined the crystal structures of the tandem C2 domains of E-Syt2 in the absence and presence of Ca2+ and analyzed their Ca2+-binding properties by nuclear magnetic resonance spectroscopy. Our data reveal an unexpected V-shaped structure with a rigid orientation between the two C 2 domains that is not substantially altered by Ca2+. The E-Syt2 C2A domain binds up to four Ca2+ ions, whereas the C2B domain does not bind Ca2+. These results suggest that E-Syt2 performs an as yet unidentified Ca2+-dependent function through its C2A domain and uncover fundamental differences between the properties of the tandem C2 domains of E-Syts and synaptotagmins. © 2014 Elsevier Ltd.

PMID: 24373768     PMCID: PMC3946263    

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